Protein fractionation by three phase partitioning (TPP) in aqueous/t-butanol mixtures
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چکیده
منابع مشابه
Purification of recombinant green fluorescent protein by three-phase partitioning.
The technique of three-phase partitioning (TPP) was used to purify the green fluorescent protein (GFP) in a single step. TPP uses a combination of ammonium sulphate and tert-butanol to precipitate proteins from their crude extracts. In the first round of TPP with 20% ammonium sulphate saturation at the ratio of crude to tert-butanol 1:1 (v/v), most of the GFP remains in the lower aqueous phase....
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 1989
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.260330213